Abstract: Leucaena glauca is a newly developed woody leguminous crop.It may serve important role to improve soil fertility and can be used as fodder for livestock and poultry.This paper reports the isolation of Rhizohlum sp.D3 from native whi-tepopinac leadtree (leucaena glauca) in Fujian Province.lt grows well in pH 4.5-5.0 acid soil and stimulates the nodulation and N2-fixing activity of Leucaena glauca.With the inoculation of Rhizohium sp. D3 the nodule number increased about 1.4-4.6 folds over that of Rhizohium sp.which introduced from Philippines.The nitrogenase activity was also increased.The bacteria number in the same volume by using solid culture method is 25 folds higher than that by liquid culture A large area demons-wtration of inoculating Rhizohium sp.DS to whitepopinac leadtree showed -that the growth rate of whitepopinac leadtree was increaded about 2 folds over the GK treatment.The effect of increasing yield was very significant.