禽多杀性巴氏杆菌荚膜亚单位疫苗对鸭的免疫研究
STUDIES ON CELL WALL CAPSULAR ANTIGEN OF PASTEURALLA MULTOCIDE IN DUCK FOWL CHOLERA IMMUNITY
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摘要: 采用2.9%NaCl溶液和SDS浸提法,从禽多杀性巴氏杆菌C48-1强毒菌株,提取一种粗荚膜抗原(CCA),荚膜形态在电镜下呈开放的C状螺圈结构或不规则形。经化学测定证明是一种含蛋白质为主的多糖蛋白复合物。用此复合物接种小白鼠、20日龄小鸭和成鸭,均无可见的全身和局部不良反应。此复合物具有免疫原性,用含蛋白质1.41~2.15mg经胸肌或翅膀皮下接种15日后,免疫8组鸭有效率平均为80%。经9批组重复效检,能获得85~100%保护率,证明免疫力稳定性可靠。测定了CCA的免疫期,可持续5~5.5个月,有效保护率为75%以上。在野外抽检了不同地区经5~6个月接种的4批免疫鸭群,其保护率与实验室结果相似。CCA在实验室和野外对蛋鸭群进行了接种试验和试用,对产蛋率均无明显影响。可用于禽霍乱暴发群,及时控制疫情。CCA可配制成禽霍乱荚膜亚单位苗,用于预防禽霍乱病,并能适用于工厂化大批量生产。Abstract: The crude capsular antigen (CCA) has been extracted by using 2.9% NaC1 solution and SDS from pasteuralla multocide strain C4S-1. Under an electronic microscope, the capeula of pasteuralla multocide was found a C spiral structure of blossom or irregular form. By chemical assay, it shows a polysaccharides-protein complexes. Mice, 20-day-old and adult ducks inoculated by the antigen are no side-effecte, and shows a good immunogenicity. With antigen containing protein 1.41-2.15mg to vaccinat 8 groups of duck through intramuscular and subcuticilar way after 15 days, the protective rate is up to 80%. Nine replicated tests have been conducted, and the protective rate is 85-100%. It shows that the efficiency is stable and reliable. CCA immune duration could sustain 5-5.5 months and the protective rate is over 75%. This has been done in the fields in several provinces of China, and the Protective rate shows similarly to that have been done in the laboratory.CCA has been vaccinated to laying ducks, and it shows no significant difference in the rate of egg-production between control group and test group both in the fields and laboratory, and it also could be used to control the disease when it is breaking out. A Subunit vaccine Can be made by CCA to prevent fowl cholera, and the vaccine could be adopted by factory and Produce in large quantities.
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[7] Bain, R .V .S, et al 1961, The antigens of pasteurela multocida-Type 1, Ⅱ, Lipopolysaccharides, Immucology, 4,122~129.
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