Regeneration of Bulblets from Tissue Culture of Oriental Hybrid Lilium,"Sorbonne"
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摘要: 通过对东方百合索蚌鳞片、鳞片叶分段诱导再生鳞茎以及鳞茎膨大的研究,建立了快繁体系。结果表明:诱导鳞片及鳞片叶产生再生鳞茎的最佳培养基都为MS+6-BA0.2(单位:mg·L-1,下同)+NAA0.2+2,4-D0.1;三种放置鳞片的方式对诱导生成的鳞茎的数量、鲜重、诱导率影响不显著;鳞片叶基部切段再生鳞茎的诱导率最高,其次为中部切段,由下向上再生鳞茎的诱导率逐渐降低;最适合诱导再生鳞茎的蔗糖浓度为30~60 g·L-1,黑暗条件下促使鳞茎膨大的蔗糖浓度以低于90 g·L-1为宜,散射光条件下促使鳞茎膨大的蔗糖浓度以低于120 g·L-1为宜。Abstract: An efficient tissue culture was developed using the bulb scale,scale′s leave segments and bulblet enlargement of the Oriental Hybrid Lilium,"Sorborne".Results showed that(a) the appropriate induction medium for regeneration of the bulblet from the bulb scale and scale′s leave segments was MS+ 0.2 mg·L-1 6-BA+NAA 0.2 mg·L-1 +0.1 mg·L-1 2,4-D;(b) there was no significant difference in quantity,induced rate and fresh weight of the regenerated bulblets resulting from the 3 putting methods;(c) the induction rate of the regenerated bulblet of scale’s leave segments was the highest in the basal,followed by the middle section,and gradually decreased from bottom to top;(d) 30-60 g·L-1 was the optimal sucrose concentration for inducing the bulblet regeneration;(e) for bulblet enlargement,the sucrose concentration should be lower than 90 g·L-1 when treated in the dark,and 120 g·L-1 when exposed under scattered light.
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Key words:
- "Sorbonne" lily /
- bulb scale /
- scale leave /
- enlargement
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[1] 徐文兴,金国良,潘荷娟.百合组织培养两次成苗[J].植物生理学通讯,1988,(3):57-59. [2] 张洁,蔡宣梅,林真,等.百合试管鳞茎诱导及膨大研究[J].福建农业学报,.2010,25(3):328-331. [3] 赵银萍,张九东,程建国.用百合叶片及其鳞茎叶片离体诱导不定芽[J].西安文理学院学报,2005,(1):21-23. [4] 龙春林,程治英,王俐,等.兰州百合器官离体培养外植体位置效应观察[J].云南植物研究,2004,26(2):221-225. [5] YOSHIJI NIMI,TSUYOSHI ONOZAWA.In vitro bulblet formation from lerf segmengts of lilies,especially Lilium rubellam baker[J].Scientia Horticulturae,1979,11:379-383. [6] 狄翠霞,安黎哲,张满效,等.西伯利亚百合器官离体培养及结鳞茎的研究[J].西北植物学报,2005,25(10):1931-1936.
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