摘要:
用0.18～0.20毫米的柑桔茎尖,在试管中进行微芽嫁接,嫁接前在切口处加1～2滴0.1ppm的激动素或玉米素,可明显提高成活率,用13～18天苗龄的枳壳砧木,嫁接成活率可达40～60%,不同梢期采的茎尖,其嫁接成活率因品种不同而有差异。再嫁接在一年生福桔实生苗上的成活率比整株移栽的高得多。黄龙病和衰退病的脱毒率分别为100%和78%。利用上述方法,已培育出13个柑桔品种的茎尖嫁接苗,其中村下雪柑、少核雪柑、天马芦柑、少核芦柑已经过鉴定,不携带黄龙病、衰退病和裂皮病病原,并定植于无病母本园。其余回蜜28号等9个品种的茎尖嫁接苗正在繁殖、鉴定中。
Abstract:
By putting a drop of O.lppm kinetin or zeatin at the cut of rootstock before grafting, the survival rate of shoot-tip grafting(STG) could come up to 40-60% by using 0.18-0.20 mm long shoot apices and 13-18 days old rootstocks. It differ significantly in different shoot-stage of shoot apices collected in different varieties. The survival rate was much higher than that of transplantation when rnicrografted seedlings in tube were directly grafted on 1-yr-old tangerine cv. Fuju (Citrus tangerina Hort. ix Tan.) seedlings 100% and 78% of elimination rates were obtained for Huanglungbin and citrus tristeza virus (CTV), respectively.Of the 13 citrus cvs. obtained from STG mentioned above, 2 sweet orange cvs. Chuangxia and Shaohe (C. sinensis var. Sekken(L.) Osbeck) and 2 mandarin cvs. Tianma and Shaohe (C.pooncnsis Tanaka) have been indexed, free from virus or virus-like diseases known, and transplanted to disease-free mother tree fundation. The other 9 cvs. such as mandarin cv. Huimi No.28 (C.unshiu Marc.) etc. have been propagating and identifying.
点击查看大图
下载:
