若干条件(因素)对芦笋花药培养诱导植株效果的影响

The Influences of Some Elements on Inducing Plants in Asparagus Officinalis Anther Culture

摘要: 芦笋花药培养以M.S作基本培养基,附加一定浓度的NAA与6-BA可诱导产生愈伤组织,再添加一定量的2,4-D可提高诱导率。愈伤组织产生多少不仅与品种有关,而且也与品种的不同植株有关。花药诱导的愈伤组织经分化培养可得到完全植株。花培植株染色体检查结果,单倍体植株很少。

Abstract: Anther culture of Asparagus was used M.S.as basic medium which added NAA and 6-BA to induce callus.Inductivity of callus could be raised by additional 2,4-D.The number of callu concerned not only cultivars but also individual plant in a cultivar.The complete plants could oh tain from the anther callus by differential cul-ture.Few haploids were observed while the chromosome of anther culture plants examined.