在盐胁迫下红萍超氧物岐化酶SOD及叶肉细胞亚显微结构的变化
Changes in Superoxide Dismute Activity and Cell LNtrastructure of Azolla During Salt Stress
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摘要: 细绿萍、小叶萍以及它们的杂种萍对盐浓度的忍受能力的顺序为:杂种萍细绿萍小叶萍,在低盐(0.2%NaCl)下,能促进红萍生长。低盐浓度胁迫下,SOD活性随盐浓度的升高而升高,但到一定盐浓度后SOD活性开始下降(小叶萍在0.4%NaCl时、细绿萍和杂种萍在0.6%NaCl时,酶活性开始下降)。SOD凝胶电泳谱带染色深浅随盐浓度的升高而加深,但到一定盐浓度后,谱带染色变浅,实验结果与SOD活性测定相对应。叶肉细胞亚显微结构的观察表明:在盐胁迫下,红萍叶片细胞受损伤的初始部位为叶绿体和线粒体,随后整个细胞的膜系统受损、混乱、以至破坏,本文并对SOD作为红萍抗盐育种的指标进行了讨论。Abstract: The result of investigation indicated that tolarance of Hybrid Azolla to salt was the best,followed by A.filiculoides and A.microphylla among three Azolla treated with different salt concentrations) during low salt concentration,Azolla were grown beneficially.The SOD activity rose higher with the increasing intensity of salt stress,but it started to reduce in certain salt concentrations (A.filiculodes and Hybrid Azolla in 0.6% NaCl,A.microphylla in 0.4% NaCl).The SOD gel electrophoresis showed the same results between SOD activity and straining intensity of enzyme bands.The ultrastructural changes of mesophyll cells showed that damage of membrance systems might be as s result of lipid reoxidation.It is suggested that SOD activity might be as an index of NaCl-resistance breeding.
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Key words:
- Azolla /
- Superoxide Dismurte /
- Salt stress /
- Mimnbrance damage /
- Cell ultrastructure
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[2] 刘鸿先等.1985.低温对不同耐寒力的黄瓜幼苗子叶各细胞器中超氧物岐化酶的影响.植物生理学报,12(3):299-251 [5] 陈缓,周玖.1983.谈谈脂质过氧化作用.生命的化学,(3):16-19 -
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