杨梅根瘤的共生固氮特性
Characteristics of Symbiotic Nitrogen Fixation by Myrica rubra
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摘要: 梅根瘤的固氮活性具有较好的持久性,不同成熟度的根瘤固氮活性以成熟瘤为最高,杨梅离体根瘤在25—30℃有较高的固氮活性,30℃时固氮活性达最高。NO3-会抑制根瘤的固氮活性和叶片的硝酸还原酶活性(NRA),不过,NO3-对有瘤杨梅苗和无瘤杨梅苗叶片NRA的影响存在一定的差异。杨梅根瘤具有吸氢酶,能有效地提高根瘤的固氮效率。Abstract: Myrica rubra is not only a subtropical tree but also a Actinomycete-nodulated plant. Root nodules of Myrica rubra have a nitrogen-fixing activity. The nitrogenase activity of exised nodules can last 4-5 hours and keep a higher activity. The nitrogen-fixing activities of nodules were determined separately, the highest activity was found in the matured nodules. The optimum temperature for nitrogen fixation of exised nodules ranged 25-30℃, the highest is at 30℃ Both nitrogenase activity of the seedlings with root nodules and nitrate reductase activity (NRA) in seedling leaves are inhibited by NO3-. However, the inhibition of NRA in the plants with or without nodules has a certain difference. The root nodules of Myrica rubra contain a hydrogen-absorbing enzyme which can increase effectively nitrogenase activity.
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Key words:
- Myrica rubra /
- Root nodule /
- Nitrogenase activity /
- Hydrogenase activity /
- Nitrate reductase activity
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[1] F. H.魏海姆,D. F.鲍勒德斯,R. M.戴威林著(中国科学院植物研究所生理生化研究室译). 1974.《植物生理学实验》.1-6.科学出版社,北京 [2] 苏梦云,周云璋. 1986.树木组织中NR测定方法.林业科技通讯,7:25-27 [3] 陈薇,张德颐.1980.植物组织中NR的提取、测定及纯化.植物生理学通讯,4,45-99
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