籽瓜花粉的液氮保存研究
The Pollen Cryopreservation of Citrullus lanutas (Thunb.) Mansfeld
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摘要: 籽瓜花粉可以在液氮中保存。用花粉作冷冻材料其冻后存活率随干燥处理时间的增加而下降;用花药作材料,其花粉冻后存活率与干燥处理时间之间的关系曲线呈抛物状。改变降温复温速率会影响花粉冻后存活率,快速降温和复温的花粉存活率都高,慢速降温或复温的花粉存活率都较低。冻后的花粉经授粉后,都能正常结实。Abstract: The pollen of Citrullus lanutas (Thunb.) Mansf eld. can be stored in liquid nitrogen (-19 6℃) . The survival rate of cryopreserved pollen decreased with the increase of drying period. When using anther as material , the relationship between the survival rate of cryopreserved pollen and drying period can be described as a parabola curve. The survival rate was also affected by cooling and warming rate, rapid cooling and warming support higher survival rate. The cryopreserved pollens may go on the normal fertilization, and the plants fruit after pollination with the pollen.
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Key words:
- Pollen /
- cryopreservation /
- Citrullus lanutas (Thunb.) Mansfeld
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[4] Bredeman, G.,Garber, K.,Harteck, P.,and Snhr, K.A.1947 Die Temperatuta-bhangigkeit der Lebensdauer Von Blutenpollen. Naturnissenschatten. 34:274
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