芦笋茎枯病菌对苯并咪唑类杀菌剂抗药性研究
Studies on Resistance of Phoma asparagi Sacc. to Benzimidazole Fungicides
-
摘要: 1989—1991年,就福建省几个芦笋产区的茎枯病菌对甲基托布津的敏感性进行测定。结果表明:长乐F1菌株和平潭L2菌株对甲基托布津表现高度抗性。在2000ppm含药量的PSA培养基上菌丝均能生长,难以测得MIC值;莆田P3菌株和福州P6菌株对甲基托布津比较敏感,在10ppm甲基托布津浓度下,菌丝完全不能生长。在种植有10多年历史,频繁使用甲基托布津的长乐湖南产区,田间抗性的病菌菌株存在比率为100%。其中F1菌株在无杀菌剂的培养基上菌丝传代培养14代后,其抗性程度仍保持稳定。长乐F1菌株和平潭L2菌株对甲基托布津、多菌灵和涕必灵苯并咪唑类杀菌剂具有交互抗药性,但对培福朗和速克灵杀菌剂的敏感性及应与莆田P3和福州P6菌株基本一致。Abstract: The sensitivity of Phoma asparagi Sacc. from diseased asparagus of different areas in Fujian to thiophanate methyl was tested during 1989 to 1991. The results showed that strains from Changle and Putian possess high resistance to thiophanate methyl. Mycelia of these resistant strains can grow on PSA medium with 2000 ug/mL thiophanate methyl and the MIC value was hardly measured. Mycelia of the sensitive strains from Putian and Fuzhou, however, failed to grow on the PSA containg 10 ug/mL thiophanate methyl. In the area of Hunan in Changle County where asparagus has been cultivated for more than ten years and thiophanate methyl was used frequently, the ratio of resistant strains was as high as 100% in the field. The thiophanate methyl-resistance of strain F1 can be maintained at least 14 generations by the means of incubation on the biocide-free PSA medium. The cross resistance of two strains F1, L2 was found among benzimidazole fungicides, such as thiophanate methy 1, carbendazim and thiabendazole. However, these resistant strains are identical to sensitive strains, and all sensitive to Procymidone and guazatine.
-
[8] Georgopulos S. G et al. 1982. FAO Plant protection Bulletin. 30(2-3):39-71,141-193
点击查看大图
计量
- 文章访问数: 2262
- HTML全文浏览量: 100
- PDF下载量: 218
- 被引次数: 0