摘要:
本文探讨用二步保护法,即冻前用低浓度DMSO抗冻剂对茎尖进行预处理,冻时用高浓度VS2液作保护剂对蕨状满江红和卡州满江红茎尖进行慢速冷冻(0.5-5.0℃/min)所取得的效果。研究表明,茎尖的生理状况,DMSO的浓度及处理时间,降温速率对茎尖冻后存活率有显著影响。将蕨状满江红冷冻到-30℃的20个不同处理中,有18个处理的53个茎尖存活,最高成活率可达100%。冷冻到-32.5℃的8个处理中,有5个处理的14个茎尖存活。
Abstract:
A new procedure, so called two-step protection for cryopreservation of Azolla meristems was developed, i. e. the meristems were pretreated by using a low concentration of DMSO, which exhibit strong penetrating ability before freezing, then treated by a high concentration of VS2, which has lower penetrating ability, as a protectant while freezing. The effect of the procedure on the meristems both of A. filiculoides and A. caroliniana at the same cooling rate was investigated. All physiological state of the meristems, concentration of DMSO, time course of protection treatment and cooling rate can effect significantly on survival rate of the meristems. 53 meristems of A. filiculoides from 18 treatments frozen at-30℃ could survive,the best survival rate reached 100%, and 14 meristems of A. filiculoides from 5 treatments frozen at-32.5 ℃ could survive as well.
点击查看大图
下载:
