R1杀雄剂诱导水稻雄性不育的扫描电镜观察(简报)
Scanning Electron Microscopic Examination on Male Sterility of Rice Induced by Gametocide R1
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摘要: 在颖花分化末期和减数分裂期,用200ppmR1或200ppmR1+400ppmE喷布籼稻品种宇B,100ppmR1+200ppmE喷布粳稻品种关东127、西海172,雄性不育率达97.7%~99.5%.扫描电镜下观察,可见喷药后花药畸形,毡绒层增厚,薄壁细胞溶脱,营养通道受到阻塞,花粉囊内不形成花粉或仅形成少量的、但半数形态败育的花粉。Abstract: High male sterility rates ranged in 97.7%-99.5% were obtained by spraying the indica rice Yu B with 200 ppm R1 or 200 ppm R1 and 400 ppm E, and by spraying japonica rice Guandong 127, Xihai 172 with 100 ppm R1 and 200 ppm E in the late stage of spikelet differentiation and meiosis Phase.In the malesterilantes treated rice, the anther deformity, thickened tapetum, thin-walled cell lysis and blocked nutrition tract were found under scanning electron microscope; the microdiodange failed to form pollen, or formed few pollen and half of which were morphologically abortive.
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Key words:
- Hybrid rice /
- Male-sterilante /
- Scanning electron microscopic observation
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[2] 徐是雄,徐雪宾.1989.稻的形态与解剖.农业出版社 [3] 田中敬一,永谷隆.1980.图说走查电子显微镜——生物作料作制法.日本朝仓出店出版
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