Abstract: Gonadotrophin has been used as a general oxytocin for toad and other frogs.Few experiments have been done on the Rana spinosa David.Since 1987, some Gonadotrophins have been used to induce the ovulation of R.spinosa.Results showed that LRH-A3 was very efficient.25 experiments were done in 4 years.The ovulation rate was 44.5%~100%, fertilized rate was 70%~99.3%.12 thousand fertilized spawn-eggs and 8 thousand hatched tadpoles have been obtained since 1987.Among which, some of them have changed into middle frogs and were active in artificial ecosystem.