灵芝菌丝体富锗效应研究初报
A Primary Repart on Enrichment of Germanlum in Mycelia of Lucid Ganoderma(Ganoderma lucidun)
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摘要: 本研究以GeO2为锗剂,通过液体深层培养获得富锗灵芝菌丝体;采用持续透析法确定了菌体提取液中存在含锗的生物大分子一有机锗。同时应用电泳技术,酶谱分析及凝胶扫描等手段初步研究了灵芝菌丝体的富锗生理效应,认为富锗作用不仅是单纯的吸收、富集作用,还包括生物转化(或同化作用)过程。此外,本文还探讨了以Ge4O2为添加剂进行富锗栽培灵芝(子实体)的可行性问题。Abstract: Germanum dioxide was applied as an additive to produce Geenriched mycelia of lucid ganoderma(Ganoderma lucidum)by liquld culture.The fact that bio macromolecule containing GeOrganic germanium was confirmed,bylasting dialysis ,in the extract liquid.In this paper,animportant conclusion was drew out that the enrichment of germanium was not only an absorption but also an assimilation.The physiologic effect of Ge-nrichment was primarily investigated by electrophores,zymographic analysis and gel scanning.Moreover,the possibility that germanium dioxide is to be used as an additive to procdue fruit bodies of lucid ganoderma was studied in this research work.
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Key words:
- Germanium dioxde /
- Lucid ganoderma /
- Mycelia /
- Ge-enrichment
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