摘要:
研究了两种抗生素对水稻种胚愈伤组织诱导和分化的影响。结果表明:供试抗生素卡那霉素(Kanamycin,Km)和氨苄青霉素(Ampicillin,Ap)对水稻种胚愈伤组织的诱导和生长都发生抑制作用,而Km比Ap的抑制作用强。不论诱导期还是分化期用Km50、100mg/kg处理,除可培养出少量白化苗外,200mg/kg以上剂量处理的均未能分化出绿苗。仅分化期用Ap处理,50～200mg/kg浓度促进了绿苗分化,但浓度达400mg/kg时则开始发生抑制作用。诱导期用Km处理的愈伤组织,在分化期其死亡率随浓度的提高而提高。而仅在分化期用Km和Ap处理的愈伤组织,虽也有不同程度的致死现象,但数量较少。Km+Ap对愈伤组织的诱导和生长具有累加抑制作用。在分化期处理,Ap可部分解除Km对绿苗分化的抑制效应。文中还讨论了Km和Ap作为检测是否导入外源基因的适宜浓度。
Abstract:
the inductions of rice embryo calli are inhibited by both Kanamycin(Km)and Ampicillin(Ap),two kinds of Antibiotics, moreover, Km is more effective than Ap, Callus induction rates are negatively and significantly correlated with Antibiotic concentrations, Antibiotics.especially Km, also inhibit the growth of calli in the induction phase, No any green seedling differentiates with Km treated either in induction phase or in differentiation phase,except that a few albinoseedlings appear in the treatments of 50 and 100 mg/kgm,Differentiations of green seedlings are promoted by50 to 200 mg/kg of Ap treated in differentiation phase.Callus death rates rise as the increases of Km concentrations treated in induction phase, Treatments withKm and Ap in differentiation phase tend to cause the death of calli in varying degrees,but death rates are lower, Km+Ap inhibit coordinately the inductions and differentiations of calli.Ap can partially remove the inhibition effects of Km on differentiation of green seedlings when treated in differentiation phase.Discussions also have been made on the proper concentrations of Km andApused to detect whether the foreign genes were introduced in.
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