甘薯品种过氧化物酶同工酶分析初报
Preliminary Research on the Analysis of Peroxidase lsoenzyme in Sweetpotato Varieties
-
摘要: 在过氧化物酶同工酶测定中甘薯植株的不同部位酶带表现有差异,而以9～11叶片之间茎组织的酶带清晰、完整和稳定,适于品种亲缘关系的分析。对88份栽培品种的茎组织进行过氧化物酶同工酶测定,共出现22条酶带,其中8条酶带表型频率均在90%以上,为甘薯茎组织过氧化物酶同工酶的稳定特征酶带。根据酶带表现把测定品种分为11类型,同一类型中的品种之间酶带表现一致,反映了较大的亲缘关系。并用模糊聚类分析法展示了各类型之间的亲缘关系。结果表明远缘杂交品种A52、优质品种金山57和C180各成一类且表现与其它品种有较大的差异,可能优质品种有特征酶带。Abstract: Different patterns of isoenzyme bands are showed in the analysis on different parts of sweetpotato.The isoenzyme bands which from the stem issue between the 9th and 11th blades are clear,complete and stable,and can be used for analysing the affinity among different varieties.We have tested the peroxidase isoenzyme of the stem tissue in 88 sweetpotato varieties.22 bands were found and 8 of them showed more than 90% apparent frequency,which are considered as the typically characteristic bands.The whole varieties were classified into 11 patterns.The varieties among one pattern have the same or similar isoenzyme bands,which revealled their affinity was more close.The affinity between different patterns was analysed by the fuzzy cluster analysis.A52,Jinshan 57 and C180 varieties are divided into seperate patterns.There are bigger gaps between them and other varieties.Maybe there are typical enzyme bands exist in the fine varieties.
-
Key words:
- Sweeetpotato varieties /
- Isoenzyme /
- Isoenzymic bands /
- Affinity /
- Fuzzy cluster analysis
-
[9] 马德华.1988.辣椒过氧化物酶同「酶研究及模糊聚类分析.华北农学报,3(3):83-90 -
点击查看大图
计量
- 文章访问数: 2261
- HTML全文浏览量: 120
- PDF下载量: 529
- 被引次数: 0
下载:
