摘要:
根据病毒粒体形态、血清学反应、寄主范围及症状,明确由福建南部地区种植的转心莲(Passiflora caerulea)和野生龙珠果(P. foetida)表现花叶症状病株分离的两个病毒分离物为黄瓜花叶病毒(Cucumber mosaic cucumovirus,CMV)的两个分离物。两分离物均能通过摩擦接种侵染供试12科55种(或品种)植物中的西番莲科、藜科、茄科、葫芦科、豆科、苋科、菊科、番杏科、夹竹桃科、玄参科、唇形科等11种的部分植物。部分提纯病毒粒体球状,直径约28～30nm.在A蛋白包被ELISA和A蛋白免疫电镜测定中,两分离物与CMV抗血清呈阳性反应,与TMV、PFWV、PGMV、PFYMV等4种抗血清呈阴性反应。
Abstract:
Two viruxes obtained from the mosaic diseases in passiflora caerulea and P. foetida in the southern Fujian,China,were identified to be two isolates of cucumber mosaic cucumovirus(CMV)based on the host range,their symptoms,serology and particle morphology.The both could infect some plants of Aizoaceae, Amaranthaceae,Apocynaceae,Chenopodiaceae,Compositae,Cucurbitaceae,Labiatae,Leguminosae, Passifloraceae, Solanaceae,Sorophulariaceae.The purified Virus were exarnined to demonstrate the isometric particles of about 28～30 nm in diameter by the electron microscope.Two virus isolates had positive reaction to CMV antiserum and negative reaction to TMV,PFwV,PGMV,PFYMV antisera in protein A coating ELISA and SPA-ISEM.
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