运用病菌毒素筛选芦笋愈伤组织突变体研究初报
The Screening of Somaclonal Mutants with Resistance to Toxin FiItrate of Phoma Asporagi Sacc. in Asparagus
-
摘要: 采用植物组织培养和病菌毒素筛选的生物技术,对芦笋愈伤组织抗病突变体进行筛选,在筛选638块愈伤组织当中,有62块愈伤组织能够生长并分化再生植株。经抗病性测定结果,表现抗病的有6株,感病的有46株。总体愈伤组织选择概率为9.4×10-3。此外,本文还就抗病材料如何利用问题进行讨论。Abstract: In vitro screening of seed-derived callus lines of Asparagus was carried out by the biotechnology of plant cell culture,with the crude toxin filtrate extracted from mycelia of P.asparagi Sacc as stress factor.Some somatic mutants resistant to Asparagus stem blight disease were obtained from 638 calli screened, 62 calli were proliferative,from which 52 regenerated plants were produced.Disease resistance of the regenerated plants to P.asporagi Sacc was examined by spraying inoculation technique, out of 52 regenerated plants, 6 plants were resistant, and 46 plants were susceptible SE of all calli was 9.4×10-3 、Some problems about the utilization of resistant plants were also discussed.
-
Key words:
- Asparagus /
- Phoma asperagi Sacc /
- Somaclonal mutant /
- Screening
点击查看大图
计量
- 文章访问数: 2284
- HTML全文浏览量: 95
- PDF下载量: 503
- 被引次数: 0