短段木灵芝优质高产菌株“赤芝6号”研究
Study on“Red Reishi No.6”,a high-yield and fine-quality strain of Ganoderma lucidum for woodlqg cultivation
-
摘要: 描述了“赤芝6号”主要栽培性状与商品性状。采用光学显微镜和扫描电镜观察了担孢子,结果表明:孢子多呈卵圆形,顶端平截或钝圆锥形,大小为6.53~8.04μm×9.55~12.56μm,孢子表面明显可见一些微小的凹陷。采用扫描电镜观察了菌肉菌丝:其骨架菌丝的骨架干直径2.6~3.95μm,分枝直径1.8~2.6μm;缠绕菌丝大多弯曲,直径1.1~1.5μm.利用聚丙烯酰胺梯度浓度凝胶电泳法分析比较了“赤芝6号”与福建其他灵芝菌株的酯酶同工酶,结果表明:“赤芝6号”有15条酶带分布在凝胶浓度8.9%~25.6%处,其酶谱特征与其他菌株明显区别。Abstract: Red Relshi No.6”with high-yield and fine-quality for woodiog cultivation was princi-pally applied and popularized by our institute in Fujian province.In this paper,the log-cultivated and economic characters were described.Electric scanning microscopy(ESM)and light rnicroscopy were used to study the basldiospore.The results showed that most of spores were ovate-obrotund with trancated or abtuse apex,their size were 6.53~8.04×9.55~12.56μm,some shallow minute hollows were evident on the spore surface.Under ESM,the trunk of skelete hyphae had a diameter of 2.6~3.95μm and the branch had a diameter of 1.8~2. 6μm,the majority of binding hyphae were tortuous and had a diarneter of 1.1~1.5μm。Easteraes zymograms of“Red Reishi No.6”and other strains in Fujian province,were analysed and compared by gradient concentration polyacry-lamide gel electrophoresis(GC-PAGE).The resuits showed that“Red Reishi No.6”had 15 bands ditributed in gelconcentration ranging from 8.9%to 25.6%,with remarkable difference from oth-er strins.
点击查看大图
计量
- 文章访问数: 2184
- HTML全文浏览量: 100
- PDF下载量: 396
- 被引次数: 0