抗坏血酸浓度对青花菜花蕾衰老的影响
Effects of Ascorbic Acid Concentrations on the Senescence of Flower Buds in Broccoli
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摘要: 青花菜采后花蕾中可溶性蛋白质和AsA(抗坏血酸)含量,SOD(超氧物歧化酶)和LOX(脂氧合酶)活性不断下降,膜脂过氧化加快,引起了衰老。外源AsA对花蕾的延衰效应依处理浓度及次数而异,100mg/LAsA稍有延衰作用,50mg/LAsA延衰作用不明显,200mg/LAsA、100mg/LAsA每日连续喷施均促进衰老。较低浓度AsA的延衰效应主要与延缓内源AsA降解、激活SOD活性有关,LOX活性降低所起的作用并不重要;较高浓度AsA促进衰老可能与引起SOD活性下降及促进Fenton反应,形成氧化力更强的,OH自由基有关。Abstract: After the harvesting of broccoli,the contents of toluble protein and ascorbic acid (AsA) together with the activities of superoxjide dismutase (SOD) and lifxjxldase (LOX) decreased constantly; lipid peroxidation increased,causing senescence in the flower buds. The effects of exogenous AsA on deferring the senescence varied with different AsA concentrations and times of treatment. 100mg/L AsA deferred the senescence slightly; 50mg/l AsA didn’t show evident deferring effect;while 200 mg/L and 100mg/L AsA with daily treatment promoted the senescence. The defer-ring effect of lower AsA concentration on the senescence was mainly related to the delay of endogenous AsA degradation and the stimulation of SOD activity. The decrease of LOX activity didn’t play an important role in deferring senescence. The promoting effect of higher AsA concentration on the senescence was probahly related to thedecrease of SOD activity,the promotion of Fenton reaction and the formation of free radical.OH which had stronger oxidizing force.
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Key words:
- Flower buds of broccoli,Senescence /
- Ascorhic acid /
- Superoxide dismutase /
- Lipxidase /
- Free radical /
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