Abstract: One virus isolated from mosaic disease of banana introduced from the PhilippInes and grown in Xiamen area,was identified as an isolate of cucumber mosaic cucumovirus subgroup Ⅱ,based on its bio-logical propertles,particle morphology and serology of the virus isolate. The virus isolate could infect 47 species (or varieties) of ten famllies from 63 species (or varieties) of 11 tested families and be also transmitted by Aphis gossypii. The thermal inactivation point for the virus isolate was 50-55℃;the dilution end point was 1:10-3-10-4, and the longevity in vitro was 24-48h. The electron microscopic examination of the purified virus particle preparations showed isometric particles of ahout 28-30nm in diameter.The antiserum obtained had a homologous titre of 1:64 in gel diffusion tests. The virus isolate had strong positive reaction to monoclonal antibodies of CMV ToRS serogroup in double antibodies sandwich enzyme-linked immunosorbent assay.