小麦幼穗离体培养筛选抗赤霉病细胞无性系的研究

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小麦幼穗离体培养筛选抗赤霉病细胞无性系的研究

刘思衡, 巫升鑫, 郭玉春, 康水英, 吴良章

文章导航 > 福建农业学报 > 1997 > 12(3): 7-10

刘思衡, 巫升鑫, 郭玉春, 康水英, 吴良章. 小麦幼穗离体培养筛选抗赤霉病细胞无性系的研究[J]. 福建农业学报, 1997, 12(3): 7-10.

引用本文:

刘思衡, 巫升鑫, 郭玉春, 康水英, 吴良章. 小麦幼穗离体培养筛选抗赤霉病细胞无性系的研究[J]. 福建农业学报, 1997, 12(3): 7-10.

Liu Siheng, Wu Shengxin, Guo Yuchun, Kang Shuiying, Wu Liangzhang. Studies on Screening Somatic Cell Lines Resistant to Scab via Explant Culture of Wheat[J]. Fujian Journal of Agricultural Sciences, 1997, 12(3): 7-10.

Citation:

Liu Siheng, Wu Shengxin, Guo Yuchun, Kang Shuiying, Wu Liangzhang. Studies on Screening Somatic Cell Lines Resistant to Scab via Explant Culture of Wheat[J]. Fujian Journal of Agricultural Sciences, 1997, 12(3): 7-10.

刘思衡, 巫升鑫, 郭玉春, 康水英, 吴良章. 小麦幼穗离体培养筛选抗赤霉病细胞无性系的研究[J]. 福建农业学报, 1997, 12(3): 7-10.

引用本文:

刘思衡, 巫升鑫, 郭玉春, 康水英, 吴良章. 小麦幼穗离体培养筛选抗赤霉病细胞无性系的研究[J]. 福建农业学报, 1997, 12(3): 7-10.

Liu Siheng, Wu Shengxin, Guo Yuchun, Kang Shuiying, Wu Liangzhang. Studies on Screening Somatic Cell Lines Resistant to Scab via Explant Culture of Wheat[J]. Fujian Journal of Agricultural Sciences, 1997, 12(3): 7-10.

Citation:

Liu Siheng, Wu Shengxin, Guo Yuchun, Kang Shuiying, Wu Liangzhang. Studies on Screening Somatic Cell Lines Resistant to Scab via Explant Culture of Wheat[J]. Fujian Journal of Agricultural Sciences, 1997, 12(3): 7-10.

小麦幼穗离体培养筛选抗赤霉病细胞无性系的研究

1.
福建农业大学农学系福州 350002

中图分类号: S512.103.53
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- 文章访问数: 2091
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出版历程
- 收稿日期: 1996-04-02
- 刊出日期: 1997-09-15

Studies on Screening Somatic Cell Lines Resistant to Scab via Explant Culture of Wheat

1.
Department of Agronomy, Fujian Agricultural University, Fuzhou 350002

摘要: 以小麦幼穗为外植体,在含赤霉菌粗毒素的培养基上进行离体培养,研究筛选抗病细胞无性系的可能性及影响因素。结果表明,品种抗性不同,其愈伤组织的诱导和绿苗分化效果也不同。外植体以发育至1～3cm长的幼穗切段为宜。赤霉菌粗毒素浓度以10%(V/V)效果较好。从感病品种组培后代中筛选出6个抗病株系。
- 小麦 /
- 幼穗 /
- 离体培养 /
- 赤霉病
Abstract: The possibility and affecting factors for screening the wheat somatic cell lines resistant to scab were studied by using explant culture of young spike on the medium containing crude toxin of F.graminearum The result showed that varieties with different resistance were different in callus induction and green plantlet differentiation; young spikes with 1-3 cm length were suitable for in vitro cultivation; the concentration of 10%(V/V) crude toxin was optimum one in the screening medium; six lines resistant to scab were obtained from scab sensitive varieties.
- Wheat /
- Young spike /
- In vitro cultivation /
- Scab

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文章访问数: 2091
HTML全文浏览量: 116
PDF下载量: 699
被引次数: 0

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